The patrons of Santa Fiora are Saints Flora and Lucilla, celebrated on 29 July in the Pieve dedicated to them.
In the hamlet of Marroneto, the «Carnevale morto» (dead Carnival) is passed down from generation to generation, an original Carnival re-enactment in which «Carnevale» is bid farewell, killed by «Quaresima» (Lent). The town holds the Monte Amiata Mercury Mines Museum, Palazzo Sforza Cesarini and the Convento della Santissima Trinità alla Selva.
