The patron saint of Sant'Ambrogio di Valpolicella is Saint Ambrose, celebrated on 7 December in the Church of Sant'Ambrogio.
The hamlets fill the year with their own feasts: the Cherry Festival and the Settembrina at Gargagnago, the ancient Saint Roch feast in the Borgo district, the Santa Maria Maddalena feast at Ponton and the Domeiara feast at Domegliara. The town also holds Villa Nichesola-Conforti and Villa Serego Alighieri.